Total signal normalization

Divides biomarker expressions by the total signal, depending on the MARGIN. This norm typically makes the most sense on a cell-by-cell level.

Usage

total_norm_region(
  region,
  MARGIN = 2,
  constant.multiplier = 100,
  to = "NormalizedData",
  from = "Data"
)

Arguments

region

A Region object, or concatenated data generated by .smapply.

MARGIN

Whether to apply the function within each biomarker (1) or across all biomarkers in each cell (2).

constant.multiplier

Constant multiplier for more readable numbers (100 means results can be interpreted like percentages).

to

Which data slot to assign the normalized data to.

from

Which data slot to pull data from.

Value

An updated Region object with normalized data added to the slot specified in to. Metadata recording information about the normalization is also added to the featureInfo.

See also

Normalize()